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KMID : 0855520120250010055
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Korean Journal of Physical Anthropology
2012 Volume.25 No. 1 p.55 ~ p.62
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Differential Expression Levels of Synaptophysin through Developmental Stages in Cerebral Cortices of Mouse Brain
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Pyeon Hee-Jang
Lee Young-Il
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Abstract
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It is well known that differentiation and growth of central nervous system are accomplished through relatively early stages of development. The formation of neural synapse indicates beginning of electrical signaling between neurons, so that may be a critical step in the differentiation of neurons as well as the development and growth of central nervous system. The purpose of this study was to investigate the differential expression levels and patterns of synaptic marker(synaptophysin) between superficial and deep layers of cerebral cortex according to the developmental stages.
We introduced immunofluorescence staining of synaptophysin combined with densitometric analysis for the morphological quantification.
The intensities of synaptophysin immuno-reactivities in deep layers of cerebral cortices were significantly higher compared to superficial layers in cerebral cortices of embryonic and neonatal mice. The significant increase of synaptophysin expression in the deep layer of cerebral cortex was mainly confined to the embryonic stage. As the expression of synaptophysin gradually decrease thereafter, the difference of expression level between superficial and deep layers could not find in the adult mice.
From this study, we could confirm indirectly through synaptophysin that synaptogenic activities in the deep layer of cerebral cortex shows unique pattern especially during the early stages of brain development. Results from this study will be helpful for understanding different patterns of synaptogenesis among the various regions of developing brain.
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KEYWORD
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Synaptogenesis, Synaptophysin, Cerebral cortex, Neural development, Mouse
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